C16 Ceramide

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

C16-Ceramide 是一种天然小分子，通过直接选择性结合激活 p53。

C16-Ceramide is a natural small molecule activating p53 through the direct and selective binding.

C16-Ceramide interacts with p53 within its core domain. p53 forms complex with natural C16-Ceramide in the cell.C16-Ceramide (2.5-50 μM; 0-48 h) strongly decreased HCT116 cell viability in a time- and concentration-dependent manner.C16-Ceramide (12 μM; 48 h) induces apoptosis through Btf (Bcl-2-associated transcription factor) in HCT116 cells.C16-ceramide (12 μM; 0-6 h) and Btf expression up-regulate p53 and BAX expression. C16-ceramide down-regulates Mdm2 expression via Btf.Cell Viability Assay Cell Line:HCT116 cells Concentration:2.5, 5, 10, 12, 20, 50 μM Incubation Time:0-48 h Result:Strongly decreased cell viability in a time- and concentration-dependent manner.Western Blot Analysis Cell Line:HCT116 cells Concentration:12 μM Incubation Time:1, 3 and 6 h Result:Increased PARP cleavage, decreased pro-caspase 3. Decreased the levels of stratifin and stathmin, increased the expression of prohibitin and Btf. RNAi-mediated Btf depletion also partially inhibited BAX expression after the treatment. Significantly decreased luciferase activity and Mdm2 protein expression levels.

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Apoptosis

p53

p53

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24696-26-2

537.9

C34H67NO3

>98% (HPLC)

In Vitro:?Ethanol 中的溶解度 : 20 mg/mL (37.18 mM; warming and heat to 60°C) DMF 中的溶解度 : 20 mg/mL (37.18 mM; 超声助溶 (<60°C))

CCCCCCCCCCCCCCCC(=O)NC(CO)C(O)C=CCCCCCCCCCCCCC

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(-20℃)

With Ice Pack

≥ 2 years